Medicare Solution India

For DNA isolation using QIAamp® and DNeasy® Kits Buffer AL* is used during DNA isolation using QIAamp and DNeasy protocols. Note that Buffer AL may form a precipitate during storage. If a precipitate has formed, incubate Buffer AL at 55°C before starting DNA isolation until the precipitate has fully dissolved. Buffer AL is stable for 1 year when stored closed at room temperature (15–25°C). Buffer AL is supplied ready for use in all QIAamp Kits and DNeasy Tissue Kits, with the exception of the DNeasy 96 Tissue Kit. Refer to the relevant handbook for instructions on the use of Buffer AL. Use of Buffer AL with the DNeasy 96 Tissue Kit When using the DNeasy 96 Blood & Tissue Kit (cat. nos. 69581 and 69582), ethanol must be added to Buffer AL to make additional Buffer AL/E: 1. Determine how much Buffer AL/E you require. Buffer AL/E is stable for 3 months when stored closed at room temperature (15–25°C). 2. Transfer the required volume of Buffer AL to a suitably sized bottle (see table below). Clearly mark the bottle as containing “Buffer AL/E”. Ensure that the new bottle is clean and that it can close tightly. 3. Add the required volume of ethanol (96–100%; see table below) to the bottle and mix thoroughly