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CELLBANKER 2 Product Code: 11914
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| Packaging Size | 500ml |
| Usage/Application | Industrial |
| Packaging Type | Bottle |
| Color | white |
| Capacity | 100ml |
| CAT No | 11914 |
| Brand | AMSBIO |
CELLBANKER 2 Product Code: 11914
Cryopreservation Medium (Serum Free)Cat # 11914 (previously [11891])Qty: 100mlExpiry Date: 3 years from manufacturing date (see label)
Cell-Freezing:For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standardfreezing protocols may be substituted.1. Examine and make sure the cell culture is free of contamination, in healthy situation and proper confluency,etc.2. Perform a cell count to determine the viability of cells3. Gently pellet the cells by centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4
oC). Remove the supernatant by
using an aspirator.4. Gently suspend the cells with CELLBANKER® 2 cryopreservation medium (1 ml for 5 x 105- 5 x 106cells).5. Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell linename, cell concentration, passage date and other essential information.6. Place the vials directly in a -80oC for storage. If necessary, transfer the frozen vials to a liquid nitrogen storagetank after the vials have been frozen for at least 24 hours.7. Optimum protocol may change with the cell types.
Cryopreservation Medium (Serum Free)Cat # 11914 (previously [11891])Qty: 100mlExpiry Date: 3 years from manufacturing date (see label)
Cell-Freezing:For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standardfreezing protocols may be substituted.1. Examine and make sure the cell culture is free of contamination, in healthy situation and proper confluency,etc.2. Perform a cell count to determine the viability of cells3. Gently pellet the cells by centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4
oC). Remove the supernatant by
using an aspirator.4. Gently suspend the cells with CELLBANKER® 2 cryopreservation medium (1 ml for 5 x 105- 5 x 106cells).5. Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell linename, cell concentration, passage date and other essential information.6. Place the vials directly in a -80oC for storage. If necessary, transfer the frozen vials to a liquid nitrogen storagetank after the vials have been frozen for at least 24 hours.7. Optimum protocol may change with the cell types.
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